KMID : 1123920150290020168
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Korean Journal of Oriental Physiology and Pathology 2015 Volume.29 No. 2 p.168 ~ p.173
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In vitro Anti-allergic Effects of Bangpungtongseong-san in Human Keratinocytes and Primary Mouse Splenocytes
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Jeong Soo-Jin
Lee Mi-Young Seo Chang-Seob Shin Hyeun-Kyoo
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Abstract
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Bangpungtongseong-san(BPTSS, Fangfengtongsheng-san in Chineses) is a traditional herbal formula comprising 18 medicinal herbs. In the present study, we performed the simultaneous analysis for four compounds of BPTSS and examined anti-allergic effects in human keratinocytes and mouse splenocytes. The column for separation of four compounds was used Luna C18 column and maintained at 40¡É. The mobile phase for gradient elution consisted of two solvent systems. The analysis was carried out at a flow rate of 1.0 mL/min with PDA detection at 254 and 280 nm. To evaluate production and expression of Th2 chemokines, ELISA and RT-PCR were conducted in tumor necrosis factor (TNF)-¥á and interferon (IFN)-¥ã-stimulated HaCaT cells with or without BPTSS or silymarin, a positive control for skin inflammation. To measure Th2 cytokines, primary mouse splenocytes were treated with BPTSS and performed ELISA for interleukin (IL)-4, 5, 13. Calibration curves were acquired with r2>0.9999. The contents of geniposide, liquiritin, baicalin, and glycyrrhizin in BPTSS were 5.06 §·/g, 7.33 §·/g, 27.56 §·/g, and 7.81 §·/g, respectively. BPTSS reduced TARC and RANTES production and mRNA expression in TNF-¥á and IFN-¥ã-treated HaCaT cells. BPTSS inhibited IL-4, 5, and 13 production in mouse splenocytes. Our data will be a helpful information to upgrade quality control and anti-allergic effects of BPTSS.
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KEYWORD
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Bangpungtongseong-san, Allergy, Th2 chemokine, Th2 cytokine
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